Nu börjar molekylärbiologerna förstå hur spikeproteinet kan skada celler.

Det är visat i studier att antikroppssvaret verkar minska efter omkring 6 månader för att det ska behövas en tredje dos.

 

Det finns fler liknande artiklar att spikeproteinet blandar sig i processer i cellkärnan. Den här artikeln ¹ visar på minskad produktion av antikroppar, det finns andra som visar på att reparation av brott på DNA-trådarna i kromosomerna hämmas ². Rent generellt så har många RNA-virus DNA-skadande egenskaper ³

Dessa artiklar visar på att det finns tidigare oförklarade anledningar till att åtminstone en del personer som injiceras med Comirnaty och andra injektioner mot Covid-19 kommer att skadas av injektionerna..

 

Jag tror fortfarande att det bästa skyddet är att försöka optimera immunförsvaret. Det innebär att man bör äta en ketogen kost baserad på animalisk föda. Betänk att 2 hg kött, fisk ägg ger tillräckligt med protein för att en vuxen att kunna tillväxa som ett spädbarn om det behövs. Sedan behövs energi och då är 2-3 hg animaliskt fett överlägset alla andra energikällor. Smör, vispgrädde, ister, tran, talg innehäller samma fetter som vi gör och är optimala med samma smälttemperatur. Vegetabiliska oljor går bort då de innehåller på tok för mycket omega-6 som är inflammationsdrivande och kan orsaka cancer. Enda undantaget är kokosolja som till del kan ersätta mjölkfett.

Livsmedelsverket älskar bönor. Men okokta bönor innehåller gifter varför de måste kokas flera gånger. Aminosyrainnehållet saknar några livsnödvändiga aminosyror så för att ersätta 2 hg kött måste man äta 8 kg kokta bönor.

Bönor och andra vegetabilier kräver odlingsbar jordbruksmark medan växtätande kor, hästar och andra kan klara sig på gräs som växer på mark som inte går att odla.

En högmjölkande ko behöver omkring 30 kg torrsubstans hö per dygn. Då samlar gräset in 88 kg CO2 varav hälften binds som rötter i marken. De flesta kolatomerna i höt går åt för att producera omkring 20 L mjölk och resten för att hålla kon vid liv under dygnet. Det blir inte många kolatomer över för att tillverka metan som kor av okunniga personer påstås spy ut tonvis av. Sedan är kor självgående gräsätare med automatgödselfunktion som inte kräver jordbruksmark medan vegetabilier kräver konstgödsel och jordbruksmark och behöver också naturgödsel som inte kan fås utan gräsätare.

 

Sedan måste man se till att få i sig tillräckligt med vitamin D3. Livsmedelsverket vet att nyfödda måste äta minst 400 IE vitamin D3 per dag för att nyfödingen inte ska drabbas av rakit eller Engelska sjukan med benskörhet före ett års ålder. D3 behövs för immunförsvaret. Som gammal barndoktor skulle jag inte drömma om att ge en vuxendos av ett läkemedel till en nyföding, barnet riskerar att dö. Inte heller kan man ge en nyföddhetsdos till en vuxen, det är overksamt.

Alltså ska en vuxen äta 400 IE/3 kg*75 kg=10 000 IE.

Det är visat i flera studier att den som har mer än 125 nmol/L vitamin D3 i blodet slipper i stort sett alla förkylningar inklusive Covid-19.

I Finland gav man nyfödingar 5 000 IE/dag under 1950-talet fram till 1964. Det blir 5 000 IE/3 kg*75 kg=125 000 IE till vuxen. Inga problem med överdosering.

Även om man blir injicerad med Covid-19-injektioner har man klart större möjlighet att överleva då man äter en ketogen kost, vitamin D3, vitamin C (askorbinsyra i livsmedelsaffären) 5-10 g/dag, 1-5 g magnesiumsulfat (Epsomsalt).

Eftersom åtminstone en del av injektionerna ger magnetiskt material i kroppen, troligtvis grafenoxid, kan man nyttja neutofila vita blodkroppar som kan spränga sig själva och kasta ut sina sammanlagt 2 m DNA per cell för att fånga mikrober och även grafenoxid i sitt DNA-nät, kallas Neutrophil extracellular traps (NETs) som kan bryta ned bakterier, svamp och till och med grafenoxid.

Så det finns hopp men denna info måste komma ut.

Det går att informera alla barn om detta så att de kan göra ett informerat val för att slippa injiceras. Föräldrarna måste båda godkänna att barnet ska injiceras. Om en förälder säger nej ska det också respekteras enligt grundlag. Väljer barnet att att säga nej trots att båda föräldrarna sagt ja ska barnets nej respekteras enligt Barnkonventionen som är svensk lag sedan 2020-01-01 och grundlagen.

Ingen får tvingas att ta någon form av medicinskt preparat enligt Regeringsformen 2 kapitlet 6 §, Patientsäkerhetslagen 6 kapitel 1 § med flera lagar. Ingen får diskrimineras enligt RF 2 kapitlet 12 §. Lag får inte brytas enligt RF 1 kapitlet 1 § 3 st.

Det är bra att lära barn litet lagar och be dem tala om för lärare och föräldrar om lärare eller sjukvårdspersonal bryter mot lag och försöker övertala något barn att injiceras. Inte heller kompisarna får påverka ett barn att ta sprutan, det är mobbning (RF 2:12) som skolan måste åtgärda enligt Skollagen, ha ett klart handlingsprogram.

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Björn Hammarskjöld
Assisterande professor i pediatrik vid Strömstas Akademi
F.d. överläkare i pediatrik
Filosofie licentiat i biokemi vid Stockholkms Universitet (1971)

 

Mer info

 

Recombinant Modified Vaccinia Virus Ankara Generating Ebola Virus-Like Particles

 

SARS–CoV–2 Spike Impairs DNA Damage Repair and Inhibits V(D)J Recombination In Vitro

10.3390/v13102056

 

Immunization with SARS Coronavirus Vaccines Leads to Pulmonary Immunopathology on Challenge with the SARS Virus

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0035421

Background Severe acute respiratory syndrome (SARS) emerged in China in 2002 and spread to other countries before brought under control. Because of a concern for reemergence or a deliberate release of the SARS coronavirus, vaccine development was initiated. Evaluations of an inactivated whole virus vaccine in ferrets and nonhuman primates and a virus-like-particle vaccine in mice induced protection against infection but challenged animals exhibited an immunopathologic-type lung disease. Design Four candidate vaccines for humans with or without alum adjuvant were evaluated in a mouse model of SARS, a VLP vaccine, the vaccine given to ferrets and NHP, another whole virus vaccine and an rDNA-produced S protein. Balb/c or C57BL/6 mice were vaccinated IM on day 0 and 28 and sacrificed for serum antibody measurements or challenged with live virus on day 56. On day 58, challenged mice were sacrificed and lungs obtained for virus and histopathology. Results All vaccines induced serum neutralizing antibody with increasing dosages and/or alum significantly increasing responses. Significant reductions of SARS-CoV two days after challenge was seen for all vaccines and prior live SARS-CoV. All mice exhibited histopathologic changes in lungs two days after challenge including all animals vaccinated (Balb/C and C57BL/6) or given live virus, influenza vaccine, or PBS suggesting infection occurred in all. Histopathology seen in animals given one of the SARS-CoV vaccines was uniformly a Th2-type immunopathology with prominent eosinophil infiltration, confirmed with special eosinophil stains. The pathologic changes seen in all control groups lacked the eosinophil prominence. Conclusions These SARS-CoV vaccines all induced antibody and protection against infection with SARS-CoV. However, challenge of mice given any of the vaccines led to occurrence of Th2-type immunopathology suggesting hypersensitivity to SARS-CoV components was induced. Caution in proceeding to application of a SARS-CoV vaccine in humans is indicated.

 

Netosis

Neutrophils are the first line of defense of the immune system against infection. Among their weaponry, they have the ability to mix and extrude their DNA and bactericidal molecules creating NET-like structures in a unique type of cell death called NETosis. This process is important in order to control extracellular infections limiting collateral damage. Its aberrant function has been implicated in several human diseases including sepsis and autoimmune disease. The purpose of the present paper is to give a general introduction to this concept.

 

Neutrophil extracellular traps (NETs) - formation and implications

PMID: 23819131

Neutrophils are cells of the immune system which freely circulate in blood vessels and are recruited to the inflammation sites when the human organism responds to microbial infections. One of the mechanisms of neutrophil action is the formation of neutrophil extracellular traps (NETs) The process of NET generation, called netosis, is a specific type of cell death, different from necrosis and apoptosis. NETs are formed by neutrophils upon contact with various bacteria or fungi as well as with activated platelets or under the influence of numerous inflammatory stimuli, and this process is associated with dramatic changes in the morphology of the cells. The main components of NETs, DNA and granular antimicrobial proteins, determine their antimicrobial properties. The pathogens trapped in NETs are killed by oxidative and non-oxidative mechanisms. On the other hand, it was also discovered that chromatin and proteases released into the circulatory system during NET formation can regulate procoagulant and prothrombotic factors and take part in clot formation in blood vessels. NETs have also been detected in lungs where they are involved in chronic inflammation processes in ALI/ARDS patients. Moreover, DNA-proteins complexes have been found in the airway fluids of cystic fibrosis patients where they can increase the viscosity of the sputum and have a negative impact on the lung functions. The DNA-complexed granular proteins and other proteins released by neutrophils during netosis lead to autoimmunity syndromes such as systemic lupus erythematosus (SLE), small-vessel vasculitis (SVV) or autoimmune diseases associated with the formation of autoantibodies against chromatin and neutrophil components. A possible involvement of NETs in metastasis is also considered.

 

https://lakartidningen.se/aktuellt/nyheter/2021/05/ny-rapport-stora-skillnader-i-hur-manga-som-dott-i-covid-19/

Sveriges överdödlighet pandemiåret 2020 var högre än grannländernas – men Sverige ligger lägre än många andra europeiska länder. Inom landet har Region Stockholm haft högst andel avlidna i covid-19 och Värmland lägst. Det visar en ny rapport från Socialstyrelsen.

 

Safety and efficacy of the ChAdOx1 nCoV-19 vaccine (AZD1222) against SARS-CoV-2: an interim analysis of four randomised controlled trials in Brazil, South Africa, and the UK

10.1016/S0140-6736(20)32661-1

 

Superparamagnetic nanoparticle delivery of DNA vaccine

The efficiency of delivery of DNA vaccines is often relatively low compared to protein vaccines. The use of superparamagnetic iron oxide nanoparticles (SPIONs) to deliver genes via magnetofection shows promise in improving the efficiency of gene delivery both in vitro and in vivo. In particular, the duration for gene transfection especially for in vitro application can be significantly reduced by magnetofection compared to the time required to achieve high gene transfection with standard protocols. SPIONs that have been rendered stable in physiological conditions can be used as both therapeutic and diagnostic agents due to their unique magnetic characteristics. Valuable features of iron oxide nanoparticles in bioapplications include a tight control over their size distribution, magnetic properties of these particles, and the ability to carry particular biomolecules to specific targets. The internalization and half-life of the particles within the body depend upon the method of synthesis. Numerous synthesis methods have been used to produce magnetic nanoparticles for bioapplications with different sizes and surface charges. The most common method for synthesizing nanometer-sized magnetite Fe3O4 particles in solution is by chemical coprecipitation of iron salts. The coprecipitation method is an effective technique for preparing a stable aqueous dispersions of iron oxide nanoparticles. We describe the production of Fe3O4-based SPIONs with high magnetization values (70 emu/g) under 15 kOe of the applied magnetic field at room temperature, with 0.01 emu/g remanence via a coprecipitation method in the presence of trisodium citrate as a stabilizer. Naked SPIONs often lack sufficient stability, hydrophilicity, and the capacity to be functionalized. In order to overcome these limitations, polycationic polymer was anchored on the surface of freshly prepared SPIONs by a direct electrostatic attraction between the negatively charged SPIONs (due to the presence of carboxylic groups) and the positively charged polymer. Polyethylenimine was chosen to modify the surface of SPIONs to assist the delivery of plasmid DNA into mammalian cells due to the polymer's extensive buffering capacity through the "proton sponge" effect.

10.1007/978-1-4939-0410-5_12

 

“Effect of calcifediol treatment and best available therapy versus best available therapy on intensive care unit admission and mortality among patients hospitalized for COVID-19: A pilot randomized clinical study”

Objective

The vitamin D endocrine system may have a variety of actions on cells and tissues involved in COVID-19 progression especially by decreasing the Acute Respiratory Distress Syndrome. Calcifediol can rapidly increase serum 25OHD concentration. We therefore evaluated the effect of calcifediol treatment, on Intensive Care Unit Admission and Mortality rate among Spanish patients hospitalized for COVID-19.

Design

Parallel pilot randomized open label, double-masked clinical trial.

Setting

University hospital setting (Reina Sofia University Hospital, Córdoba Spain.)

Participants

76 consecutive patients hospitalized with COVID-19 infection, clinical picture of acute respiratory infection, confirmed by a radiographic pattern of viral pneumonia and by a positive SARS-CoV-2 PCR with CURB65 severity scale (recommending hospital admission in case of total score > 1).

Procedures

All hospitalized patients received as best available therapy the same standard care, (per hospital protocol), of a combination of hydroxychloroquine (400 mg every 12 h on the first day, and 200 mg every 12 h for the following 5 days), azithromycin (500 mg orally for 5 days. Eligible patients were allocated at a 2 calcifediol:1 no calcifediol ratio through electronic randomization on the day of admission to take oral calcifediol (0.532 mg), or not. Patients in the calcifediol treatment group continued with oral calcifediol (0.266 mg) on day 3 and 7, and then weekly until discharge or ICU admission. Outcomes of effectiveness included rate of ICU admission and deaths.

Results

Of 50 patients treated with calcifediol, one required admission to the ICU (2%), while of 26 untreated patients, 13 required admission (50 %) p value X2 Fischer test p < 0.001. Univariate Risk Estimate Odds Ratio for ICU in patients with Calcifediol treatment versus without Calcifediol treatment: 0.02 (95 %CI 0.002−0.17). Multivariate Risk Estimate Odds Ratio for ICU in patients with Calcifediol treatment vs Without Calcifediol treatment ICU (adjusting by Hypertension and T2DM): 0.03 (95 %CI: 0.003-0.25). Of the patients treated with calcifediol, none died, and all were discharged, without complications. The 13 patients not treated with calcifediol, who were not admitted to the ICU, were discharged. Of the 13 patients admitted to the ICU, two died and the remaining 11 were discharged.

Conclusion

Our pilot study demonstrated that administration of a high dose of Calcifediol or 25-hydroxyvitamin D, a main metabolite of vitamin D endocrine system, significantly reduced the need for ICU treatment of patients requiring hospitalization due to proven COVID-19. Calcifediol seems to be able to reduce severity of the disease, but larger trials with groups properly matched will be required to show a definitive answer.

10.1016/j.jsbmb.2020.105751

 

“Effect of calcifediol treatment and best available therapy versus best available therapy on intensive care unit admission and mortality among patients hospitalized for COVID-19: A pilot randomized clinical study”

 

 

18 Reasons I Won't Be Getting a Covid Vaccine

https://www.citizensjournal.us/18-reasons-i-wont-be-getting-a-covid-vaccine/

 

 

Comparison of nonhomologous end joining and homologous recombination in human cells

Zhiyong Mao, Michael Bozzella, Andrei Seluanov, and Vera Gorbunova^*

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2695993/

The two major pathways for repair of DNA double-strand breaks (DSBs) are homologous recombination (HR) and nonhomologous end joining (NHEJ). HR leads to accurate repair, while NHEJ is intrinsically mutagenic. To understand human somatic mutation it is essential to know the relationship between these pathways in human cells. Here we provide a comparison of the kinetics and relative contributions of HR and NHEJ in normal human cells. We used chromosomally integrated fluorescent reporter substrates for real-time in vivo monitoring of the NHEJ and HR. By examining multiple integrated clones we show that the efficiency of NHEJ and HR is strongly influenced by chromosomal location. Furthermore, we show that NHEJ of compatible ends (NHEJ-C) and NHEJ of incompatible ends (NHEJ-I) are fast processes, which can be completed in approximately 30 min, while HR is much slower and takes 7h or longer to complete. In actively cycling cells NHEJ-C is twice as efficient as NHEJ-I, and NHEJ-I is three times more efficient than HR. Our results suggest that NHEJ is a faster and more efficient DSB repair pathway than HR.

Keywords: DNA repair, Normal human fibroblasts, Nonhomologous end joining, Homologous recombination

 

 

 

22 Scientists Publish Paper Claiming The PCR Test Is “Useless” For Detecting COVID-19 Cases

What Happened: A recent publication titled “Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR” recently published in the Journal Eurosurveillance has come under fire by 22 scientists/independent researchers. The publication claims that the RT-qPCR tests used for detecting COVID-19 is quite robust and a useful tool, but the independent publication presents a number of scientific […]

https://www.collective-evolution.com/2020/12/02/22-scientists-publish-paper-claiming-the-pcr-test-is-useless-for-detecting-covid-19-cases/

 

 

A comparison between virion RNA of measles virus and some other paramyxoviruses

10.1007/BF02121717

 

 

Vitamin D Status in Hospitalized Patients with SARS-CoV-2 Infection

AbstractBackground. The role of vitamin D status in COVID-19 patients is a matter of debate.Objectives. To assess serum 25-hydroxyvitamin D (25OHD) levels in ho

10.1210/clinem/dgaa733

 

 

MicroRNA

https://en.wikipedia.org/w/index.php?title=MicroRNA&oldid=1049351812

A microRNA (abbreviated miRNA) is a small single-stranded non-coding RNA molecule (containing about 22 nucleotides) found in plants, animals and some viruses, that functions in RNA silencing and post-transcriptional regulation of gene expression. miRNAs function via base-pairing with complementary sequences within mRNA molecules. As a result, these mRNA molecules are silenced, by one or more of the following processes: (1) cleavage of the mRNA strand into two pieces, (2) destabilization of the mRNA through shortening of its poly(A) tail, and (3) less efficient translation of the mRNA into proteins by ribosomes.miRNAs resemble the small interfering RNAs (siRNAs) of the RNA interference (RNAi) pathway, except miRNAs derive from regions of RNA transcripts that fold back on themselves to form short hairpins, whereas siRNAs derive from longer regions of double-stranded RNA. The human genome may encode over 1900 miRNAs, although more recent analysis suggests that the number is closer to 2,300. However, only about 500 human microRNAs represent bona fide miRNA in the manually curated miRNA gene database MirGeneDB.miRNAs are abundant in many mammalian cell types and as extracellular circulating miRNAs. Circulating miRNAs are released into body fluids including blood and cerebrospinal fluid and have the potential to be available as biomarkers in a number of diseases. MiRNAs appear to target about 60% of the genes of humans and other mammals. Many miRNAs are evolutionarily conserved, which implies that they have important biological functions. For example, 90 families of miRNAs have been conserved since at least the common ancestor of mammals and fish, and most of these conserved miRNAs have important functions, as shown by studies in which genes for one or more members of a family have been knocked out in mice.

 

 

Immunoregulation by 1,25-dihydroxyvitamin D3: Basic concepts

10.1016/j.jsbmb.2005.06.002

 

Global, regional, and national comparative risk assessment of 84 behavioural, environmental and occupational, and metabolic risks or clusters of risks for 195 countries and territories, 1990–2017